表達方式: A DNA sequence encoding the TEV Protease （NP_062908） （Gly 2038- Gly 2280） was fused with polyhistidine tag at the N-terminus, with a Ser 2256 Asn mutation
種屬:Human
表達宿主: E.Coli
純度:> 95%, as determined by SDS-PAGE
內(nèi)毒素:< ﹤1.0 EU per 1μg cytokine as determined by the LAL method.
穩(wěn)定性:Samples are stable for up to twelve months from date of receipt -70°C
預測N端: Met 1
分子量:The recombinant human consists of 251 amino acids and has a predicted molecular mass of 28.6 kDa. As estimated in SDS-PAGE under reducing conditions.
緩沖液: Supplied as a 0.2μm filtered solution of 50mM Tris, 10%Sucrose,5mM DTT pH8.0
SDS-PAGE:
儲存方法:Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and usage. Avoid repeated freeze-thaw cycles. Reconstitution:Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Protein Description
TEV Protease is the 241 amino acid, 27 kDa catalytic domain of the nuclear inclusion a （NIa） protease from tobacco etch virus. As a 3C-type protease, this enzyme specifically recognizes and processes at the consensus sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly in cis and in trans, and cleavage occurs between the Gln and Gly residues. Unlike factor Xa, enteropeptidase or thrombin, has not been found to cleave at unintended sites even when present at high concentration. Due to its high specificity and high activity rate within a wide range of pH and ionic strength conditions, is an optimal and useful reagent for removing affinity tags from genetically engineered fusion proteins. However, a serious drawback of is its autocatalytic activity at S2256 to generate a truncated enzyme with greatly diminished activity. Therefore, mutated s have been produced for improved stability.